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Coliform Medium, Modified (MCM)

[所属分类:培养基配方] [发布时间:2021-11-12] [发布人:网站管理员2] [阅读次数:] [返回]

Coliform Medium, Modified

(MCM)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Lactose..............................................................20.0g

Tris(hydroxymethyl)aminomethane buffer.......12.1g

Proteose peptone No. 3 .....................................10.0g

Yeast extract........................................................6.0g

Bile salts No. 3....................................................1.0g

Sodium lauryl sulfate..........................................1.0g

Sodium deoxycholate..........................................0.1g

Bromcresol Purple solution .......................... 10.0mL

pH 6.8 ± 0.2 at 25°C

Bromcresol Purple Solution:

Composition per 100.0mL:

Bromcresol Purple ............................................0.35g

NaOH (0.1N solution)..................................... 2.0mL

Preparation of Bromcresol Purple Solution:

Combine Bromcresol Purple and NaOH solution.

Mix thoroughly. Bring volume to 100.0mL with dis

tilled/deionized water. Filter sterilize.

Preparation of Medium: Add components, ex

cept Bromcresol Purple solution, to distilled/deion

ized water and bring volume to 990.0mL. Mix

thoroughly. Add 10.0mL of Bromcresol Purple solu

tion. Mix thoroughly. Adjust pH to 6.8 with 1N

NH4OH. Distribute into flasks in 95.0mL volumes.

Autoclave for 25 min at 15 psi pressure–121°C.

Use: For the isolation and cultivation of coliform mi

croorganisms from yogurt.