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Colonization Medium

[所属分类:培养基配方] [发布时间:2021-11-12] [发布人:网站管理员2] [阅读次数:] [返回]
Colonization Medium

山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com

Composition per 700.0mL:

Mannose..............................................................1.0g
Pancreatic digest of gelatin.................................0.2g
Brain heart, solids from infusion ......................0.08g
Peptic digest of animal tissue............................0.08g
NaCl..................................................................0.07g
Glucose .............................................................0.04g
Na2HPO4 ...........................................................0.03g
Bile salts No. 3....................................................0.1g
Dulbecco’s phosphate-buffered saline ........ 700.0mL
pH 7.4 ± 0.2 at 25°C
Dulbecco’s Phosphate-Buffered Saline:
Composition per liter:
NaCl....................................................................8.0g
Na2HPO4·7H2O.................................................2.16g
KCl......................................................................0.2g
KH2PO4...............................................................0.2g
CaCl2...................................................................0.1g
MnCl2·6H2O .......................................................0.1g
Preparation of Dulbecco’s Phosphate-Buff
ered Saline: Add components to distilled/deionized
water and bring volume to 1.0L. Mix thoroughly.
Preparation of Medium: Combine components.
Mix thoroughly. Filter sterilize. Aseptically distrib
ute into sterile tubes or flasks.
Use: For the differentiation of enterotoxigenic Es
cherichia coli from foods based on the HeLa cell test
for colonization.
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