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Congo Red Agar (CR Agar)

[所属分类:培养基配方] [发布时间:2021-11-12] [发布人:网站管理员2] [阅读次数:] [返回]

Congo Red Agar

(CR Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

GC agar base............................................... 890.0mL

Hemoglobin solution .................................. 100.0mL

Supplement solution ..................................... 10.0mL

Congo Red (0.01% solution) .......................... 0.1mL

pH 7.2 ± 0.2 at 25°C

GC Agar Base:

Composition per 890.0mL:

Agar ..................................................................10.0g

Pancreatic digest of casein..................................7.5g

Peptic digest of animal tissue .............................7.5g

NaCl....................................................................5.0g

K2HPO4...............................................................4.0g

Cornstarch...........................................................1.0g

KH2PO4...............................................................1.0g

Preparation of GC Agar Base: Add components

to distilled/deionized water and bring volume to

890.0mL. Mix thoroughly. Gently heat until boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 45°–50°C.

Hemoglobin Solution:

Composition per 100.0mL:

Hemoglobin ........................................................2.0g

Preparation of Hemoglobin Solution: Add he

moglobin to distilled/deionized water and bring vol

ume to 100.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 50°C.

Congo Red Solution:

Composition per 100.0mL:

Congo Red ........................................................0.01g

Preparation of Congo Red Solution: Add Congo

Red to 100.0mL of distilled/deionized water. Mix thor

oughly. Autoclave for 15 min at 15 psi pressure–

121°C.

Supplement Solution:

Composition per liter:

Glucose ...........................................................100.0g

L-Cysteine·HCl..................................................25.9g

L-Glutamine.......................................................10.0g

L-Cystine .............................................................1.1g

Adenine...............................................................1.0g

Nicotinamide adenine dinucleotide ..................0.25g

Vitamin B12 .........................................................0.1g

Thiamine pyrophosphate.....................................0.1g

Guanine·HCl .....................................................0.03g

Fe(NO3)3·6H2O.................................................0.02g

p-Aminobenzoic acid......................................0.013g

Thiamine·HCl ..................................................3.0mg

Preparation of Supplement Solution: Add

components to distilled/deionized water and bring

volume to 1.0L. Mix thoroughly. Filter sterilize.

Source: The supplement solution IsoVitaleX® en

richment is available from BD Diagnostic Systems.

This enrichment may be replaced by supplement VX

from BD Diagnostic Systems.

Preparation of Medium: To 890.0mL of sterile,

cooled GC agar base, aseptically add 100.0mL of ster

ile, cooled hemoglobin solution, 10.0mL of sterile

supplement solution, and 0.1mL of sterile Congo Red

solution. Mix thoroughly. Pour into sterile Petri dishes.

Use: For the isolation and differentiation of virulent

and avirulent strains of Shigella, Vibrio cholerae, and

Escherichia coli.

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Congo Red Agar (CR Agar)