培养基配方

您现在的位置：网站首页 >> 培养基配方

Congo Red BHI Agarose Medium (CRBHO Medium) (BAM M41)

[所属分类:培养基配方] [发布时间:2021-11-12] [发布人:网站管理员2] [阅读次数:] [返回]

Congo Red BHI Agarose Medium

(CRBHO Medium)

(BAM M41)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agarose .............................................................12.0g

Pancreatic digest of gelatin...............................14.5g

Brain heart, solids from infusion ........................6.0g

Peptic digest of animal tissue..............................6.0g

NaCl....................................................................5.0g

Glucose ...............................................................3.0g

Na2HPO4 .............................................................2.5g

MgCl2..................................................................1.0g

Congo Red solution ...................................... 20.0mL

pH 7.4 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 15 min

at 15 psi pressure–121°C. Pour into sterile Petri dish

es in 20.0mL volumes.

Congo Red Solution:

Composition per 100.0mL:

Congo Red ...................................................375.0mg

Preparation of Congo Red Solution: Add Con

go Red to 100.0mL of distilled/deionized water. Mix

thoroughly. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 25°C.

Use: For the isolation, cultivation, and detection of

virulent strains of Yersinia enterocolitica.