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Congo Red Magnesium Oxalate Agar (CRMOX Agar)

[所属分类:培养基配方] [发布时间:2021-11-12] [发布人:网站管理员2] [阅读次数:] [返回]

Congo Red Magnesium

Oxalate Agar

(CRMOX Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Solution 1.................................................... 825.0mL

Solution 2...................................................... 80.0mL

Solution 3...................................................... 80.0mL

Solution 4...................................................... 10.0mL

Solution 5........................................................ 5.0mL

pH 7.3 ± 0.2 at 25°C

Solution 1:

Composition per 825.0mL:

Pancreatic digest of casein................................15.0g

Agar ..................................................................15.0g

Papaic digest of soybean meal............................5.0g

NaCl....................................................................5.0g

pH 7.3 ± 0.2 at 25°C

Preparation of Solution 1: Add components to

distilled/deionized water and bring volume to

825.0mL. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Do not overheat.

Solution 2:

Composition per liter:

MgCl2·6H2O .....................................................50.8g

Preparation of Solution 2: Add MgCl2·6H2O to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Autoclave for 15 min at 15 psi pres

sure–121°C.

Solution 3:

Composition per liter:

Sodium oxalate .................................................33.2g

Preparation of Solution 3: Add sodium oxalate

to distilled/deionized water and bring volume to

1.0L. Mix thoroughly. Autoclave for 15 min at 15 psi

pressure–121°C.

Solution 4:

Composition per 100.0mL:

D-Galactose .......................................................20.0g

Preparation of Solution 4: Add D-galactose to

distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Filter sterilize.

Solution 5:

Composition per 10.0mL:

Congo Red ..........................................................0.1g

Preparation of Solution 5: Add Congo Red to

distilled/deionized water and bring volume to

10.0mL. Mix thoroughly. Autoclave for 15 min at 15

psi pressure–121°C.

Preparation of Medium: Aseptically combine

80.0mL of sterile solution 2, 80.0mL of sterile solu

tion 3, 10.0mL of sterile solution 4, and 5.0mL of

sterile solution 5. Mix thoroughly. Warm to 50°C.

Add this mixture to 825.0mL of cooled, sterile solu

tion 1. Mix thoroughly. Pour into sterile Petri dishes.

Use: For the cultivation and identification of patho

genic serotypes of Yersinia enterocolitica. For the de

termination of whether Yersinia strains contain the

Yersinia virulence plasmid.

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Congo Red Magnesium Oxalate Agar (CRMOX Agar)