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Cooked Meat Medium, Modified

[所属分类:培养基配方] [发布时间:2021-11-15] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Cooked meat medium .......................................66.0g

Solution A.......................................................... 1.0L

pH 6.8 ± 0.2 at 25°C

Solution A:

Composition per liter:

Pancreatic digest of casein................................10.0g

Glucose ...............................................................2.0g

Soluble starch......................................................1.0g

Sodium thioglycolate ..........................................1.0g

Neutral Red (1% aqueous).............................. 5.0mL

Preparation of Solution A: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat until dissolved.

Preparation of Medium: Add 1.0g of cooked meat

medium to each of 66 test tubes. Add 15.0mL of solu

tion A to each test tube. Allow meat particles to rehy

drate. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation of a variety of anaerobic mi

croorganisms.