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Cooked Meat Medium, Modified (BAM M43)

[所属分类:培养基配方] [发布时间:2021-11-15] [发布人:网站管理员2] [阅读次数:] [返回]

Cooked Meat Medium, Modified

(BAM M43)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per tube:

Cooked meat medium .........................................1.0g

Diluent ............................................................... 1.0L

pH 6.8 ± 0.2 at 25°C

Cooked Meat Medium:

Composition per 481g:

Beef heart........................................................454.0g

Proteose peptone...............................................20.0g

NaCl....................................................................5.0g

Glucose ...............................................................2.0g

Source: Cooked meat medium is available in dehy

drated form from BD Diagnostic Systems.

Diluent:

Composition per liter:

Pancreatic digest of casein................................10.0g

Glucose ...............................................................2.0g

Soluble starch......................................................1.0g

Sodium thioglycolate..........................................1.0g

Neutral Red (1% aqueous).............................. 5.0mL

Preparation of Diluent: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat until dissolved.

Preparation of Medium: Add 1.0g of dehydrated

cooked meat medium and 15.0mL diluent to 20 ×

150mm test tubes. Let meat particles rehydrate. Gen

tly heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C.