培养基配方

您现在的位置：网站首页 >> 培养基配方

Cornmeal and V8 Juice Agar (ATCC Medium 309)

[所属分类:培养基配方] [发布时间:2021-11-15] [发布人:网站管理员2] [阅读次数:] [返回]

Cornmeal and V8 Juice Agar

(ATCC Medium 309)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ....................................................................7.5g

CaCO3 .................................................................3.0g

Cornmeal Extract ........................................ 800.0mL

V8 Juice ...................................................... 200.0mL

pH 5.6–6.0 at 25°C

Cornmeal Extract:

Composition per 800.0mL:

Yellow cornmeal ...............................................50.0g

Preparation of Cornmeal Infusion: Add 50.0g of

yellow Cornmeal to 800.0mL of water. Leave in re

frigerator overnight. Heat Cornmeal mixture to 60°C

for 1 hr. Filter out Cornmeal through cheese cloth.

Bring volume back to 800.0mL.

Preparation of Medium: Combine components.

Mix thoroughly. Gently heat and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Pour

into sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation and maintenance of fungi.