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Cornmeal Agar, Quarter-Strength (ATCC Medium 2221)

[所属分类:培养基配方] [发布时间:2021-11-15] [发布人:网站管理员2] [阅读次数:] [返回]

Cornmeal Agar, Quarter-Strength

(ATCC Medium 2221)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Cornmeal Infusion ...................................... 250.0mL

pH 5.6–6.0 at 25°C

Cornmeal Infusion:

Composition per liter:

Yellow cornmeal ...............................................50.0g

Preparation of Cornmeal Infusion: Add cornmeal

to distilled/deionized water and bring volume to

1.0L. Gently heat and bring to boiling. Simmer for 10

min. Filter through cheesecloth. Return volume to

1.0 liter.

Preparation of Medium: Add agar to 250.0mL

cornmeal infusion and bring volume to 1.0L with dis

tilled/deionized water. Gently heat and bring to boil

ing. Autoclave for 15 min at 15 psi pressure–121°C.

Pour into sterile Petri dishes or distribute into sterile

tubes.

Use: For the cultivation and maintenance of fungi.