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Crystal Violet Pectate Medium (CVP Medium)

[所属分类:培养基配方] [发布时间:2021-11-15] [发布人:网站管理员2] [阅读次数:] [返回]

Crystal Violet Pectate Medium

(CVP Medium)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sodium polypectate ............................................9.0g

Agar ....................................................................2.0g

NaNO3 ................................................................1.0g

NaOH (1N solution)........................................ 4.5mL

CaCl2·H2O (10% solution).............................. 3.0mL

Crystal Violet (0.075% solution) .................... 1.0mL

Sodium lauryl sulfate (10% solution)............. 0.5mL

pH 7.2 ± 0.2 at 25°C

Preparation of Medium: In a preheated blender,

add 500.0mL of boiling distilled/deionized water and

the components, except sodium polypectate and so

dium lauryl sulfate solution. Blend at high speed for

15 sec. Continue blending at low speed and slowly

add 9.0g of sodium polypectate. Pour the incomplete

medium into a 2L flask and add 0.5mL of sodium

lauryl sulfate solution. Mix thoroughly. Cap flask

with an aluminum foil seal rather than cotton. Auto

clave for 25 min at 15 psi pressure–121°C. Pour me

dium quickly into sterile Petri dishes. Allow plates to

dry at 25°C for 48 hr before use.

Use: For the cultivation of pectinolytic microorgan

isms, such as Erwinia species, from foods.