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CS Vitamin B12 Agar

[所属分类:培养基配方] [发布时间:2021-11-15] [发布人:网站管理员2] [阅读次数:] [返回]

CS Vitamin B12 Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glucose .............................................................20.0g

K2SO4................................................................20.0g

Agar ..................................................................15.0g

Sodium acetate..................................................12.0g

Vitamin assay casamino acids...........................10.0g

Papaic digest of soybean meal............................5.0g

Sodium thioglycolate ..........................................1.7g

K2HPO4...............................................................1.0g

KH2PO4...............................................................1.0g

Ribonucleic acid .................................................1.0g

Sorbitan monooleate complex ............................1.0g

MgSO4·7H2O ......................................................0.4g

DL-Tryptophan.....................................................0.2g

L-Cystine .............................................................0.2g

FeSO4 ................................................................0.02g

MgSO4·7H2O ....................................................0.02g

NaCl..................................................................0.02g

Adenine sulfate ...............................................0.018g

Guanine·HCl ...................................................0.012g

Uracil ................................................................0.01g

Xanthine............................................................0.01g

Pyridoxal..........................................................4.0mg

Pyridoxine........................................................4.0mg

Calcium pentothenate ......................................2.0mg

Niacin...............................................................2.0mg

Riboflavin ........................................................2.0mg

Thiamine·HCl ..................................................2.0mg

Folic acid..........................................................1.0mg

Biotin ............................................................... 1.0μg

Lactobacillus leichmannii suspension ............ 1.0mL

pH 6.2 ± 0.1 at 25°C

Preparation of Medium: Add components, ex

cept Lactobacillus leichmannii suspension, to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C. Inoculate medium with 1.0mL of Lacto

bacillus leichmannii suspension. Mix thoroughly.

Pour into sterile 150mm Petri dishes in 50.0mL vol

umes. Allow agar surface to dry before using.

Use: For the microbiological assay of vitamin B12 by

the cup plate or disk method using Lactobacillus le

ichmannii as the test microorganism.

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CS Vitamin B12 Agar