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CYA Agar with Arginine and p-Aminobenzoic Acid (ATCC Medium 2033)

[所属分类:培养基配方] [发布时间:2021-11-15] [发布人:网站管理员2] [阅读次数:] [返回]

CYA Agar with Arginine

and p-Aminobenzoic Acid

(ATCC Medium 2033)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sucrose..............................................................30.0g

Agar ..................................................................15.0g

Yeast extract........................................................5.0g

NaNO3.................................................................3.0g

K2HPO4...............................................................1.0g

KCl......................................................................0.5g

MgSO4·7H2O......................................................0.5g

Arginine ..............................................................0.2g

FeSO4·7H2O......................................................0.01g

p-Aminobenzoic acid.......................................1.0mg

pH 7.3 ± 0.2 at 25°C

Sucrose Solution:

Composition per 100.0mL:

Sucrose..............................................................30.0g

Preparation of Sucrose Solution: Add sucrose

to distilled/deionized water and bring volume to

100.0mL. Mix thoroughly. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 50°C.

Preparation of Medium: Add components, except

sucrose solution, to distilled/deionized water and bring

volume to 900.0mL. Mix thoroughly. Autoclave for 15

min at 15 psi pressure–121°C. Cool to 50°C. Asepti

cally add sterile sucrose solution. Mix thoroughly.

Pour into sterile Petri dishes or leave in tubes.

Use: For the isolation and cultivation of heat-resis

tant filamentous fungi (molds) from foods.