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Czapek Yeast Extract Agar

[所属分类:培养基配方] [发布时间:2021-11-15] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sucrose..............................................................30.0g

Agar ..................................................................15.0g

Yeast extract........................................................5.0g

NaNO3.................................................................3.0g

K2HPO4...............................................................1.0g

MgSO4·7H2O ......................................................0.5g

KCl......................................................................0.5g

FeSO4·7H2O......................................................0.01g

Trace metal solution........................................ 1.0mL

pH 7.3 ± 0.2 at 25°C

Trace Metal Solution:

Composition per 100.0mL:

ZnSO4·7H2O .......................................................1.0g

CuSO4·5H2O .......................................................0.5g

Preparation of Trace Metal Solution: Add

components to 100.0mL distilled/deionized water.

Mix thoroughly.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Adjust pH to 6.2. Gently heat and

bring to boiling. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C. Pour into

sterile Petri dishes or leave in tubes.

Use: For the cultivation and maintenance of a vari

ety of fungal and bacterial species that can use nitrate

as sole nitrogen source.