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DCLS Agar (Deoxycholate Citrate Lactose Sucrose Agar)

[所属分类:培养基配方] [发布时间:2021-11-15] [发布人:网站管理员2] [阅读次数:] [返回]

DCLS Agar

(Deoxycholate Citrate

Lactose Sucrose Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................12.0g

Sodium citrate·3H2O.........................................10.5g

Lactose................................................................5.0g

Na2S2O3 ..............................................................5.0g

Sucrose................................................................5.0g

Pancreatic digest of casein..................................3.5g

Peptic digest of animal tissue .............................3.5g

Beef extract.........................................................3.0g

Sodium deoxycholate..........................................2.5g

Neutral Red.......................................................0.03g

pH 7.2 ± 0.1 at 25°C

Source: This medium is available as a premixed pow

der from BD Diagnostic Systems and Oxoid.

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat while stirring and bring to boil

ing. Do not overheat. Do not autoclave. Pour into sterile

Petri dishes in 20.0mL volumes.

Use: For the selective isolation of Salmonella spe

cies, Shigella species, and Vibrio species.