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Decarboxylase Basal Medium with NaCl (BAM M44)

[所属分类:培养基配方] [发布时间:2021-11-15] [发布人:网站管理员2] [阅读次数:] [返回]

Decarboxylase Basal Medium with NaCl

(BAM M44)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Peptone or gelysate .............................................5.0g

Yeast extract........................................................3.0g

Glucose ...............................................................1.0g

Bromcresol Purple ............................................0.02g

pH 6.5 ± 0.2 at 25°C

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Adjust pH so that it will be 6.5 ± 0.2 after

sterilization. Distribute into 16 × 150mm screw

capped tubes in 5.0mL volumes. Autoclave medium

with loosely capped tubes for 10 min at 15 psi pressure–

121°C. Screw the caps on tightly for storage and after

inoculation.

Use: For the cultivation and differentiation of Vibrio

spp. based on their ability to decarboxylate the amino

acid. As the basal medium for arginine broth, lysine

broth, and ornithine broth. Bacteria that decarboxy

late arginine, lysine, or ornithine turn the medium

turbid purple. The unsupplemented decarboylase

basal medium is used as a control.