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Decarboxylase Base, Møller

[所属分类:培养基配方] [发布时间:2021-11-15] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Amino acid........................................................10.0g

Beef extract.........................................................5.0g

Peptone................................................................5.0g

Glucose ...............................................................0.5g

Bromcresol Purple ............................................0.01g

Cresol Red........................................................5.0mg

Pyridoxal..........................................................5.0mg

Mineral oil................................................... 200.0mL

pH 6.0 ± 0.2 at 25°C

Source: This medium is available as a premixed pow

der from BD Diagnostic Systems.

Preparation of Medium: Add components, except

mineral oil, to distilled/deionized water and bring vol

ume to 1.0L. For amino acid, use L-arginine, L-lysine, or

L-ornithine. Mix thoroughly. Distribute into screw

capped tubes in 5.0mL volumes. Autoclave medium

and mineral oil separately for 15 min at 15 psi pressure–

121°C. After inoculation, overlay medium with 1.0mL

of sterile mineral oil per tube.

Use: For the cultivation and differentiation of bacte

ria based on their ability to decarboxylate the amino

acid. Bacteria that decarboxylate arginine, lysine, or

ornithine turn the medium turbid purple.