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Decarboxylase Medium, Ornithine Modified

[所属分类:培养基配方] [发布时间:2021-11-15] [发布人:网站管理员2] [阅读次数:] [返回]

Decarboxylase Medium,

Ornithine Modified

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

L-Ornithine ........................................................10.0g

Meat peptone ......................................................5.0g

Yeast extract........................................................3.0g

Bromcresol Purple solution ............................ 5.0mL

pH 5.5 ± 0.2 at 25°C

Bromcresol Purple Solution:

Composition per 100.0mL:

Bromcresol Purple ..............................................0.2g

Ethanol.......................................................... 50.0mL

Preparation of Bromcresol Purple Solution:

Add Bromcresol Purple to ethanol. Mix thoroughly.

Bring volume to 100.0mL with distilled/deionized wa

ter. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat until dissolved. Adjust pH to

5.5 with HCl or NaOH. Distribute into screw-capped

tubes. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and differentiation of bacte

ria based on their ability to decarboxylate ornithine.

Bacteria that decarboxylate ornithine turn the medi

um turbid purple.