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Demi-Fraser Broth

[所属分类:培养基配方] [发布时间:2021-11-15] [发布人:网站管理员2] [阅读次数:] [返回]

Demi-Fraser Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..................................................................20.0g

Tryptose ............................................................10.0g

Na2HPO4 .............................................................9.6g

Beef extract.........................................................5.0g

Yeast extract........................................................5.0g

LiCl .....................................................................3.0g

KH2PO4.............................................................1.35g

Esculin ................................................................1.0g

Acriflavin·HCl ...............................................12.5mg

Nalidixic acid.................................................10.0mg

Ferric ammonium citrate supplement ........... 10.0mL

pH 7.2 ± 0.2 at 25°C

Source: This medium is available as a premixed pow

der and supplement from BD Diagnostic Systems.

Ferric Ammonium Citrate Supplement:

Composition per 10.0mL:

Ferric ammonium citrate.....................................0.5g

Preparation of Ferric Ammonium Citrate

Supplement: Add ferric ammonium citrate to dis

tilled/deionized water and bring volume to 10.0mL.

Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except

ferric ammonium citrate supplement, to distilled/deion

ized water and bring volume to 990.0mL. Mix thor

oughly. Autoclave for 15 min at 15 psi pressure–121°C.

Aseptically add 10.0mL of sterile ferric ammonium cit

rate supplement. Mix thoroughly. Aseptically distribute

into sterile tubes or flasks.

Use: For the cultivation of Listeria species from

foods.