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Dichloran Rose Bengal Chloramphenicol Agar (DRBC Agar) (BAM M183)

[所属分类:培养基配方] [发布时间:2021-11-16] [发布人:网站管理员2] [阅读次数:] [返回]

Dichloran Rose Bengal

Chloramphenicol Agar

(DRBC Agar)

(BAM M183)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Glucose .............................................................10.0g

Peptone ...............................................................5.0g

KH2PO4...............................................................1.0g

MgSO4·7H2O......................................................0.5g

Chloramphenicol.................................................0.1g

Dichloran solution .......................................... 1.0mL

Rose Bengal solution ...................................... 0.5mL

pH 5.6 ± 0.2 at 25°C

Dichloran Solution:

Composition per 10.0mL:

Dichloran (2,6-dichloro-4-nitroaniline)...........0.2.0g

Preparation of Dichloran Solution: Add dichlo

ran to 10.0mL of distilled/deionized water. Mix thor

oughly.

Rose Bengal Solution:

Composition per 10.0mL:

Rose Bengal........................................................0.5g

Preparation of Rose Bengal Solution: Add

Rose Bengal to 10.0mL of distilled/deionized water.

Mix thoroughly.

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Mix thor

oughly. Autoclave for 15 min at 15 psi pressure–121°C.

The final pH should be 5.6. Temper in a water bath at

45°C. Pour into sterile Petri dishes or distribute into

sterile tubes. This medium is light sensitive and should

be stored in a dark, cool place until used. It is intended

for spread plates only.

Use: For the analysis of food samples containing

spreader molds, e.g., Mucor and Rhizopus. The

dichloran and Rose Bengal slow down the growth of

fast-growing fungi, thus allowing the detection of

other fungi with slower growth rates.