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Dichloran-Glycerol Agar (DG18)

[所属分类:培养基配方] [发布时间:2021-11-16] [发布人:网站管理员2] [阅读次数:] [返回]

Dichloran-Glycerol Agar

(DG18)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glycerol ..........................................................220.0g

Agar ..................................................................15.0g

Glucose .............................................................10.0g

Peptone ...............................................................5.0g

KH2PO4...............................................................1.0g

MgSO4 ................................................................0.5g

Chloramphenicol.................................................0.2g

Dichloran .........................................................2.0mg

pH 5.6 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Preparation of Medium: Add components, except

glycerol, to distilled/deionized water and bring volume

to 800.0mL. Mix thoroughly. Gently heat and bring to

boiling. Cool to 50°C. Add 220.0g of glycerol. Mix

thoroughly. Bring volume to 1.0L with distilled/deion

ized water. Autoclave for 15 min at 15 psi pressure–

121°C. The final pH should be 5.6. Pour into sterile Pet

ri dishes or distribute into sterile tubes.

Use: A selective low water activity (aw ) medium for

xerophilic molds from dried and semi-dried foods

(dried fruits, spices, confectionery, cereals, nuts,

dried meat, and dried fish products) as well as a gen

eral purpose medium for counting yeasts and molds

in foodstuffs.