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Dichloran 18% Glycerol Agar (DG18 Agar) (BAM M184)

[所属分类:培养基配方] [发布时间:2021-11-16] [发布人:网站管理员2] [阅读次数:] [返回]

Dichloran 18% Glycerol Agar

(DG18 Agar)

(BAM M184)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Glycerol ..........................................................220.0g

Agar ..................................................................15.0g

Glucose .............................................................10.0g

Peptone................................................................5.0g

KH2PO4...............................................................1.0g

MgSO4·7H2O ......................................................0.5g

Dichloran .........................................................2.0mg

Chloramphenicol ................................................0.1g

pH 5.6 ± 0.2 at 25°C

Preparation of Medium: Add components, except

glycerol, to distilled/deionized water and bring volume

to 950.0mL. Mix thoroughly. Gently heat and bring to

boiling. Cool to 50°C. Add 220.0g of glycerol. Mix

thoroughly. Bring volume to 1.0L with distilled/deion

ized water. Autoclave for 15 min at 15 psi pressure–

121°C. The final pH should be 5.6 and the final aw

should be 0.955. Pour into sterile Petri dishes or distrib

ute into sterile tubes.

Use: Used as a general purpose medium for the enu

meration of molds from foods. For the enumeration

and isolation of xerophilic molds from dried and

semidried foods. This medium is preferred when the

aw of the analyzed food is 0.95 or lower. The low wa

ter activity of this medium reduces interference by

bacteria and fast-growing fungi.