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DNase Test Agar with Toluidine Blue

[所属分类:培养基配方] [发布时间:2021-11-16] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Pancreatic digest of casein................................10.0g

Peptic digest of animal tissue............................10.0g

NaCl....................................................................5.0g

Deoxyribonucleic acid ........................................2.0g

Toluidine Blue.....................................................0.1g

pH 7.3 ± 0.2 at 25°C

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat while stirring and bring to boil

ing. Distribute into tubes or flasks. Autoclave for 15 min

at 13 psi pressure–118°C. Pour into sterile Petri dishes

or leave in tubes.

Use: For the differentiation of microorganisms, es

pecially Staphylococcus species and Serratia marce

scens, based on their production of deoxyribo

nuclease.