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Dorset Egg Medium

[所属分类:培养基配方] [发布时间:2021-11-16] [发布人:网站管理员2] [阅读次数:] [返回]

Dorset Egg Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Homogenized whole egg ............................ 950.0mL

Glycerol ........................................................ 50.0mL

pH 6.8–7.4 at 25°C

Source: This medium is available as a prepared medi

um from BD Diagnostic Systems.

Homogenized Whole Egg:

Composition per liter:

Whole eggs ......................................................18–24

Preparation of Homogenized Whole Egg:

Use fresh eggs, less than 1 week old. Scrub the shells

with soap. Let stand in a soap solution for 30 min.

Rinse in running water. Soak eggs in 70% ethanol for 15

min. Break the eggs into a sterile container. Homoge

nize by shaking. Filter through four layers of sterile

cheesecloth into a sterile graduated cylinder. Measure

out 1.0L.

Preparation of Medium: Filter sterilize glycerol.

Combine glycerol and homogenized whole egg. Mix

thoroughly. Distribute into sterile screw-capped tubes.

Place tubes in a slanted position. Inspissate at 85°C

(moist heat) for 45 min.

Use: For the maintenance of Mycobacterium spe

cies.