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Double-Strength Crude Lactobacillus Medium

[所属分类:培养基配方] [发布时间:2021-11-16] [发布人:网站管理员2] [阅读次数:] [返回]

Double-Strength Crude

Lactobacillus Medium

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1025.0mL:

Yeast extract (Basamine) ..................................20.0g

Sucrose..............................................................20.0g

Casein hydrolysate............................................15.0g

Potassium acetate................................................3.0g

Histidine·HCl·H2O..............................................2.0g

Ascorbic acid ......................................................1.0g

Pyridoxamine·HCl ..........................................33.0μg

Salts solution A............................................. 20.0mL

Salts solution B............................................... 5.0mL

pH 5.4 ± 0.2 at 25°C

Salts Solution A:

Composition per liter:

K2HPO4·3H2O ..................................................16.5g

KH2PO4·H2O ....................................................16.5g

Preparation of Salts A: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Autoclave for 15 min at 15 psi pressure–

121°C.

Salts Solution B:

Composition per liter:

MgSO4·7H2O......................................................8.0g

FeSO4·7H2O........................................................0.4g

NaCl....................................................................0.4g

HCl, concentrated ........................................... 0.1mL

MnSO4·H2O.................................................... 0.1mL

Preparation of Salts B: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Autoclave for 15 min at 15 psi pressure–

121°C.

Preparation of Medium: Add components, except

salts solution A and salts solution B, to distilled/deion

ized water and bring volume to 975.0mL. Mix thor

oughly. Autoclave for 15 min at 15 psi pressure–121°C.

Aseptically add 20.0mL of sterile salts solution A and

5.0mL of sterile salts solution B. Mix thoroughly. Ad

just pH to 5.4. Mix thoroughly. Aseptically distribute

into sterile tubes or flasks.

Use: For the cultivation of Lactobacillus species.