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Double-Strength Crude Medium for Lactobacillus

[所属分类:培养基配方] [发布时间:2021-11-16] [发布人:网站管理员2] [阅读次数:] [返回]

Double-Strength Crude Medium

for Lactobacillus

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sucrose..............................................................20.0g

Yeast extract......................................................20.0g

Casein hydrolysate............................................15.0g

Potassium acetate................................................3.0g

Histidine·HCl ......................................................2.0g

Ascorbic acid ......................................................1.0g

Pyridoxamine·HCl ......................................... 33.0μg

Salts A........................................................... 20.0mL

Salts B............................................................. 5.0mL

pH 5.4 ± 0.2 at 25°C

Salts A:

Composition per 100.0mL:

KH2PO4·H2O.....................................................16.5g

K2HPO4·3H2O...................................................16.5g

Preparation of Salts A: Add components to dis

tilled/deionized water and bring volume to 100.0mL.

Mix thoroughly.

Salts B:

Composition per 100.0mL:

MgSO4·7H2O ......................................................8.0g

FeSO4·7H2O........................................................0.4g

MnSO4·H2O........................................................0.4g

NaCl....................................................................0.4g

HCl, concentrated ........................................... 0.1mL

Preparation of Salts B: Add components to dis

tilled/deionized water and bring volume to 100.0mL.

Mix thoroughly.

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Adjust

pH to 5.4 with acetic acid. Distribute into tubes or

flasks. Autoclave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and maintenance of Lacto

bacillus species.