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Doyle and Roman Enrichment Medium

[所属分类:培养基配方] [发布时间:2021-11-16] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Pancreatic digest of casein................................10.0g

Peptic digest of animal tissue............................10.0g

NaCl....................................................................5.0g

Sodium succinate ................................................3.0g

Yeast extract........................................................2.0g

Glucose ...............................................................1.0g

NaHSO3 ..............................................................0.1g

L-Cysteine·HCl·H2O ...........................................0.1g

Horse blood, lysed ........................................ 70.0mL

Antibiotic solution ........................................ 10.0mL

pH 7.0 ± 0.2 at 25°C

Antibiotic Solution:

Composition per 10.0mL:

Cycloheximide..................................................0.05g

Vancomycin ....................................................0.015g

Trimethoprim lactate........................................5.0mg

Polymyxin B ..............................................200,000U

Preparation of Antibiotic Solution: Add com

ponents to distilled/deionized water and bring volume

to 10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, except

antibiotic solution and horse blood, to distilled/deion

ized water and bring volume to 920.0mL. Mix thor

oughly. Gently heat and bring to boiling. Autoclave for

15 min at 15 psi pressure–121°C. Cool to 45°–50°C.

Aseptically add sterile antibiotic solution and horse

blood. Mix thoroughly. Aseptically distribute into ster

ile flasks in 90.0–100.0mL volumes.

Use: For the cultivation and enrichment of Campy

lobacter species from foods.