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DRBC Agar (Dichloran Rose Bengal Chloramphenicol Agar)

[所属分类:培养基配方] [发布时间:2021-11-16] [发布人:网站管理员2] [阅读次数:] [返回]

DRBC Agar

(Dichloran Rose Bengal

Chloramphenicol Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Glucose .............................................................10.0g

Peptone ...............................................................5.0g

KH2PO4...............................................................1.0g

MgSO4·7H2O......................................................0.5g

Rose Bengal....................................................0.025g

Dichloran ........................................................0.002g

Chloramphenicol solution............................. 10.0mL

pH 5.6 ± 0.2 at 25°C

Source: This medium is available as a premixed pow

der from Oxoid.

Chloramphenicol Solution:

Composition per 10.0mL:

Chloramphenicol.................................................0.1g

Preparation of Chloramphenicol Solution:

Add chloramphenicol to distilled/deionized water and

bring volume to 10.0mL. Mix thoroughly. Filter steril

ize.

Preparation of Medium: Add components, except

chloramphenicol solution, to distilled/deionized water

and bring volume to 990.0mL. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15 psi

pressure–121°C. Cool to 45°–50°C. Aseptically add

sterile chloramphenicol solution. Mix thoroughly. Pour

into sterile Petri dishes or distribute into sterile tubes.

Use: For the isolation, cultivation, and enumeration

of yeasts and molds associated with food spoilage.