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Duncan-Strong Sporulation Medium, Modified (DS Sporulation Medium, Modified) (Sporulation Medium, Modified) (BAM M45)

[所属分类:培养基配方] [发布时间:2021-11-16] [发布人:网站管理员2] [阅读次数:] [返回]

Duncan-Strong

Sporulation Medium, Modified

(DS Sporulation Medium, Modified)

(Sporulation Medium, Modified)

(BAM M45)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Proteose peptone...............................................15.0g

Na2HPO4·7H2O.................................................10.0g

Raffinose.............................................................4.0g

Yeast extract........................................................4.0g

Sodium thioglycolate ..........................................1.0g

pH 7.8 ± 0.1 at 25°C

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Distribute

into tubes or flasks. Autoclave for 15 min at 15 psi pres

sure–121°C. Adjust pH to 7.8 with filter-sterilized

0.66M Na2CO3. Pour into sterile Petri dishes or leave in

tubes.

Use: For the cultivation and induction of sporulation

of Clostridium perfringens.