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Egg Yolk Agar

[所属分类:培养基配方] [发布时间:2021-11-17] [发布人:网站管理员2] [阅读次数:] [返回]

Egg Yolk Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Proteose peptone No. 2 .....................................40.0g

Agar ..................................................................25.0g

Na2HPO4 .............................................................5.0g

Glucose ...............................................................2.0g

NaCl....................................................................2.0g

KH2PO4...............................................................1.0g

MgSO4·7H2O ......................................................0.1g

Egg yolk emulsion ...................................... 100.0mL

Hemin solution................................................ 1.0mL

pH 7.6 ± 0.2 at 25°C

Hemin Solution:

Composition per 100.0mL:

Hemin..................................................................0.5g

NaOH (1N solution)...................................... 20.0mL

Preparation of Hemin Solution: Add hemin to

20.0mL of 1N NaOH solution. Mix thoroughly.

Bring volume to 100.0mL with distilled/deionized

water.

Egg Yolk Emulsion:

Composition:

Chicken egg yolks..................................................11

Whole chicken egg...................................................1

Preparation of Egg Yolk Emulsion: Soak eggs

with 1:100 dilution of saturated mercuric chloride so

lution for 1 min. Crack eggs and separate yolks from

whites. Mix egg yolks with 1 chicken egg.

Preparation of Medium: Add components, ex

cept egg yolk emulsion, to distilled/deionized water

and bring volume to 900.0mL. Mix thoroughly. Gen

tly heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 45°–50°C. Aseptical

ly add sterile egg yolk emulsion. Mix thoroughly.

Pour into sterile Petri dishes.

Use: For the isolation, cultivation, and differentiation

of Clostridium species and some other anaerobic bacte

ria.