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Egg Yolk Agar, Modified

[所属分类:培养基配方] [发布时间:2021-11-17] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................20.0g

Pancreatic digest of casein................................15.0g

Vitamin K1 ........................................................10.0g

NaCl....................................................................5.0g

Papaic digest of soybean meal............................5.0g

Yeast extract........................................................5.0g

L-Cystine .............................................................0.4g

Hemin...............................................................5.0mg

Egg yolk emulsion ...................................... 100.0mL

Source: This medium is available as a prepared me

dium from BD Diagnostic Systems.

Egg Yolk Emulsion:

Composition:

Chicken egg yolks..................................................11

Whole chicken egg ..................................................1

Preparation of Egg Yolk Emulsion: Soak eggs

with 1:100 dilution of saturated mercuric chloride so

lution for 1 min. Crack eggs and separate yolks from

whites. Mix egg yolks with 1 chicken egg.

Preparation of Medium: Add components, ex

cept egg yolk emulsion, to distilled/deionized water

and bring volume to 900.0mL. Mix thoroughly. Gen

tly heat and bring to boiling. Autoclave for 15 min at

15 psi pressure–121°C. Cool to 45°–50°C. Aseptical

ly add sterile egg yolk emulsion. Mix thoroughly.

Pour into sterile Petri dishes.

Use: For the isolation, cultivation, and differentia

tion of Clostridium species and some other anaerobic

bacteria.