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Egg Yolk Emulsion

[所属分类:培养基配方] [发布时间:2021-11-17] [发布人:网站管理员2] [阅读次数:] [返回]

Egg Yolk Emulsion

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 100mL:

Sterile saline.................................................. 70.0mL

Egg yolk........................................................ 30.0mL

Source: Sterile egg yolk emulsion is available from

Fluka, Sigma-Aldrich.

Preparation of Medium: Use fresh eggs, less than

1 week old. Scrub the shells with soap. Let stand in a

soap solution for 30 min. Rinse in running water. Soak

eggs in 70% ethanol for 15 min or soak eggs with

1:100 dilution of saturated mercuric chloride solution

for 1 min. Crack eggs and separate yolks from

whites, placing egg yolks into a sterile container. Use

enough eggs to produce at least 30.0mL egg yolk.

Homogenize by shaking. Add 0.9g NaCl to distilled/

deionized water and bring volume to 100.0mL. Ster

ilze the saline solution by filtration or by autoclaving

for 15 min at 15 psi pressure–121°C. If autoclaving

is used, cool to 25°C. Aseptically add 30.0mL ho

mogenized egg yolks to 70.0mL of sterile saline so

lution. Mix thoroughly.

Use: Sterile stabilized emulsion of egg yolk recom

mended for use in various culture media.