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Egg Yolk Emulsion

[所属分类:培养基配方] [发布时间:2021-11-17] [发布人:网站管理员2] [阅读次数:] [返回]

Egg Yolk Emulsion

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 100mL:

NaCl..................................................................0.45g

Egg yolk........................................................ 50.0mL

Preparation of Medium: Use fresh eggs, less than

1 week old. Scrub the shells with soap. Let stand in a

soap solution for 30 min. Rinse in running water. Soak

eggs in 70% ethanol for 15 min. or soak eggs with

1:100 dilution of saturated mercuric chloride solution

for 1 min. Crack eggs and separate yolks from

whites, placing egg yolks into a sterile container. Use

enough eggs to produce at least 50.0mL egg yolk.

Homogenize by shaking. Add 0.45g NaCl to dis

tilled/deionized water and bring volume to 50.0mL.

Sterilze the saline solution by filtration or by auto

claving for 15 min at 15 psi pressure–121°C. If auto

claving is used, cool to 25°C. Aseptically add

50.0mL homogenized egg yolks to 50.0mL of the

sterile NaCl solution. Mix thoroughly.

Use: Sterile stabilized emulsion of egg yolk recom

mended for use in various culture media.