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EMB Agar, Modified (Eosin Methylene Blue Agar, Modified)

[所属分类:培养基配方] [发布时间:2021-11-17] [发布人:网站管理员2] [阅读次数:] [返回]

EMB Agar, Modified

(Eosin Methylene Blue Agar, Modified)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Lactose..............................................................10.0g

Pancreatic digest of gelatin...............................10.0g

K2HPO4...............................................................2.0g

Eosin Y ...............................................................0.4g

Methylene Blue...............................................0.065g

pH 6.8 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Distribute

into tubes or flasks. Autoclave for 15 min at 15 psi pres

sure–121°C. Cool to 60°C. Shake medium to oxidize

methylene blue. Pour into sterile Petri dishes. Swirl

flask while pouring plates to distribute precipitate.

Use: For the isolation, cultivation, and differentia

tion of Gram-negative enteric bacteria based on lac

tose fermentation. Bacteria that ferment lactose,

especially the coliform bacterium Escherichia coli,

appear as colonies with a green metallic sheen or

blue-black to brown color. Bacteria that do not fer

ment lactose appear as colorless or transparent, light

purple colonies.