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Endo Agar

[所属分类:培养基配方] [发布时间:2021-11-17] [发布人:网站管理员2] [阅读次数:] [返回]

Endo Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Lactose..............................................................10.0g

Peptic digest of animal tissue ...........................10.0g

K2HPO4...............................................................3.5g

Na2SO3................................................................2.5g

Basic Fuchsin......................................................0.5g

pH 7.4 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Caution: Basic Fuchsin is a potential carcinogen

and care must be taken to avoid inhalation of the

powdered dye and contact with the skin.

Preparation of Medium: Add components to dis

tilled/deionized water and bring volume to 1.0L. Mix

thoroughly. Gently heat and bring to boiling. Auto

clave for 15 min at 15 psi pressure–121°C. Cool to

45°–50°C. Pour into sterile Petri dishes. Swirl flask

while pouring plates to keep precipitate in suspension.

Protect from the light.

Use: For the selective isolation, cultivation, and dif

ferentiation of coliform and other enteric microorgan

isms based on their ability to ferment lactose. Lactose

fermenting bacteria appear as dark red colonies with a

gold metallic sheen. Lactose-nonfermenting bacteria

appear as colorless or translucent colonies.