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Endo Agar

[所属分类:培养基配方] [发布时间:2021-11-17] [发布人:网站管理员2] [阅读次数:] [返回]

Endo Agar

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................10.0g

Lactose..............................................................10.0g

Peptic digest of animal tissue............................10.0g

K2HPO4...............................................................3.5g

Na2SO3................................................................2.5g

Basic Fuchsin solution.................................... 4.0mL

pH 7.5 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Caution: Basic Fuchsin is a potential carcinogen

and care must be taken to avoid inhalation of the

powdered dye and contact with the skin.

Basic Fuchsin Solution:

Composition per 10.0mL:

Basic Fuchsin......................................................1.0g

Ethanol (95% solution) ................................. 10.0mL

Preparation of Basic Fuchsin Solution: Add

Basic Fuchsin to 10.0mL of ethanol. Mix thoroughly.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 45°–50°C. Pour into sterile Petri dishes. Swirl

flask while pouring plates to keep precipitate in sus

pension. Protect from the light.

Use: For the selective isolation, cultivation, and dif

ferentiation of coliform and other enteric microorgan

isms based on their ability to ferment lactose. Lactose

fermenting bacteria appear as dark red colonies with a

gold metallic sheen. Lactose-nonfermenting bacteria

appear as colorless or translucent colonies.