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Endo Agar, LES (m-Endo Agar, LES)

[所属分类:培养基配方] [发布时间:2021-11-17] [发布人:网站管理员2] [阅读次数:] [返回]

Endo Agar, LES

(m-Endo Agar, LES)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................10.0g

Lactose................................................................9.4g

Tryptose ..............................................................7.5g

NaCl....................................................................3.7g

Peptone................................................................3.7g

Pancreatic digest of casein..................................3.7g

K2HPO4...............................................................3.3g

Na2SO3................................................................1.6g

Yeast extract........................................................1.2g

KH2PO4...............................................................1.0g

Sodium deoxycholate..........................................0.1g

Sodium lauryl sulfate........................................0.05g

Basic Fuchsin solution.................................... 8.0mL

pH 7.2 ± 0.2 at 25°C

Caution: Basic Fuchsin is a potential carcinogen

and care must be taken to avoid inhalation of the

powdered dye and contact with the skin.

Basic Fuchsin Solution:

Composition per 10.0mL:

Basic Fuchsin......................................................1.0g

Ethanol (95% solution)................................. 10.0mL

Preparation of Basic Fuchsin Solution: Add

Basic Fuchsin to 10.0mL of ethanol. Mix thoroughly.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Autoclave for 15 min at 15 psi pressure–121°C. Cool

to 45°–50°C. Pour into sterile Petri dishes. Swirl

flask while pouring plates to keep precipitate in sus

pension. Protect from the light.

Use: For the cultivation and enumeration of coliform

bacteria from water by the membrane filter method.