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Endo Broth (m-Endo Broth)

[所属分类:培养基配方] [发布时间:2021-11-17] [发布人:网站管理员2] [阅读次数:] [返回]

Endo Broth

(m-Endo Broth)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Lactose..............................................................12.5g

Peptone .............................................................10.0g

NaCl....................................................................5.0g

Pancreatic digest of casein..................................5.0g

Peptic digest of animal tissue .............................5.0g

K2HPO4...........................................................4.375g

Na2SO3................................................................2.1g

Yeast extract........................................................1.5g

KH2PO4...........................................................1.375g

Basic Fuchsin....................................................1.05g

Sodium deoxycholate..........................................0.1g

Ethanol (95% solution)................................. 20.0mL

pH 7.2 ± 0.1 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Caution: Basic Fuchsin is a potential carcinogen

and care must be taken to avoid inhalation of the

powdered dye and contact with the skin.

Preparation of Medium: Add ethanol to approx

imately 900.0mL of distilled/deionized water. Add

remaining components. Bring volume to 1.0L with

distilled/deionized water. Mix thoroughly. Gently

heat and bring to boiling. Rapidly cool broth below

45°C. Do not autoclave. Use 1.8–2.0mL for each fil

ter pad. Protect from the light. Prepare broth freshly.

Use: For the cultivation and enumeration of coliform

bacteria from water by the membrane filter method.