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Malonate Broth

[所属分类:培养基配方] [发布时间:2021-11-30] [发布人:网站管理员2] [阅读次数:] [返回]

Malonate Broth

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sodium malonate ................................................3.0g

NaCl....................................................................2.0g

(NH4)2SO4...........................................................2.0g

K2HPO4...............................................................0.6g

KH2PO4...............................................................0.4g

Bromthymol Blue ...........................................0.025g

pH 6.7 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C. Avoid in

troduction of carbon and nitrogen from other sources.

Use: For the cultivation and differentiation of

coliforms and other enteric organisms, particularly

Enterobacter and Escherichia, based on their ability

to utilize malonate as the sole carbon source and

ammonium sulfate as the sole nitrogen source. Mal

onate-utilizing organisms turn the medium blue.