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Malonate Broth, Ewing Modified (BAM M92)

[所属分类:培养基配方] [发布时间:2021-11-30] [发布人:网站管理员2] [阅读次数:] [返回]

Malonate Broth, Ewing Modified

(BAM M92)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Sodium malonate ................................................3.0g

NaCl....................................................................2.0g

(NH4)2SO4...........................................................2.0g

Yeast extract........................................................1.0g

Glucose .............................................................0.25g

K2HPO4...............................................................0.6g

KH2PO4...............................................................0.4g

Bromthymol Blue ...........................................0.025g

pH 6.7 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C.

Use: For the cultivation and differentiation of

coliforms and other enteric organisms, particularly

Enterobacter and Escherichia, based on their ability

to utilize malonate as a carbon source and ammo

nium sulfate as a nitrogen source. The small amount

of yeast extract and glucose encourages the growth of

some organisms that may be distressed or fail to

respond. Malonate-utilizing organisms turn the

medium blue.