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Malt Extract Yeast Extract 40% Glucose Agar (MY40G)
[所属分类:培养基配方] [发布时间:2021-11-30] [发布人:网站管理员2] [阅读次数:] [返回]
Malt Extract Yeast Extract
40% Glucose Agar
(MY40G)
山东拓普生物工程有限公司 培养基配方 http://www.topbiol.com
Composition per liter:
Glucose ...........................................................400.0g
Agar ..................................................................12.0g
Malt extract powder..........................................12.0g
Yeast extract........................................................3.0g
pH 5.5 ± 0.2 at 25°C
Preparation of Medium: Add components, ex
cept glucose, to 550.0mL of distilled/deionized wa
ter. Mix thoroughly. Gently heat and bring to boiling.
Bring volume to 600.0mL with distilled/deionized
water. While the solution is still hot, add the glucose
all at once while stirring to avoid formation of lumps.
Autoclave for 30 min at 0 psi pressure–100°C.
Use: For the isolation and cultivation of osmotoler
ant microorganisms from foods.