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Mannitol Egg Yolk Polymyxin Agar (MYP Agar) (BAM M95)

[所属分类:培养基配方] [发布时间:2021-12-1] [发布人:网站管理员2] [阅读次数:] [返回]

Mannitol Egg Yolk

Polymyxin Agar

(MYP Agar)

(BAM M95)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

D-Mannitol ........................................................10.0g

Peptone..............................................................10.0g

NaCl..................................................................10.0g

Beef extract.........................................................1.0g

Egg yolk emulsion, 50%............................... 50.0mL

Polymyxin B ................................................. 10.0mL

Phenol Red, 1% in 95% ethanol ..................... 2.5mL

pH 7.2 ± 0.1 at 25°C

Source: Available as a prepared medium from BD

Diagnostic Systems.

Egg Yolk Emulsion, 50%:

Composition per 100.0mL:

Chicken egg yolks..................................................11

Whole chicken egg ..................................................1

NaCl (0.9% solution).................................... 80.0mL

Preparation of Egg Yolk Emulsion, 50%:

Soak eggs with 1:100 dilution of saturated mercuric

chloride solution for 1 min. Crack eggs and separate

yolks from whites. Mix egg yolks with 1 chicken egg.

Measure 50.0mL of egg yolk emulsion and add to

50.0mL of 0.9% NaCl solution. Mix thoroughly. Fil

ter sterilize. Warm to 45°–50°C.

Polymyxin B Solution:

Composition per 50.0mL:

Polymyxin B ..............................................500,000U

Preparation of Polymyxin B Solution: Add

polymyxin B to distilled/deionized water and bring

volume to 50.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components—ex

cept egg yolk emulsion, 50%, and polymyxin B solu

tion—to distilled/deionized water and bring volume

to 940.0mL. Mix thoroughly. Gently heat and bring

to boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 45°–50°C. Aseptically add 50.0mL

of sterile egg yolk emulsion, 50%, and 10.0mL of

sterile polymyxin B solution. Mix thoroughly. Pour

into sterile Petri dishes.

Use: For the cultivation and enumeration of Bacillus

cereus from foods.

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Mannitol Egg Yolk Polymyxin Agar (MYP Agar) (BAM M95)