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Mannitol Maltose Agar (BAM M96)

[所属分类:培养基配方] [发布时间:2021-12-1] [发布人:网站管理员2] [阅读次数:] [返回]

Mannitol Maltose Agar

(BAM M96)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

NaCl..................................................................20.0g

Agar ..................................................................13.0g

D-Mannitol ........................................................10.0g

Maltose .............................................................10.0g

Beef extract.........................................................5.0g

Papaic digest of soybean meal............................5.0g

Polypeptone ........................................................5.0g

Dye stock solution, 1000× .............................. 1.0mL

pH 7.8 ± 0.2 at 25°C

Dye Stock Solution, 1000X:

Bromthymol Blue ...............................................4.0g

Cresol Red ..........................................................4.0g

Ethanol, 95%............................................... 100.0mL

Preparation of Dye Stock Solution, 1000X:

Add Bromthymol Blue and Cresol Red to 100.0mL

of ethanol. Mix thoroughly.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Adjust to pH 7.8. Gently heat and

bring to boiling. Distribute into tubes or flasks. Auto

clave for 15 min at 15 psi pressure–121°C. Pour into

sterile Petri dishes or leave in tubes.

Use: For the cultivation of Vibrio species from

foods.