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Medium for Lactobacilli (ATCC Medium 980)

[所属分类:培养基配方] [发布时间:2021-12-1] [发布人:网站管理员2] [阅读次数:] [返回]

Medium for Lactobacilli

(ATCC Medium 980)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................20.0g

Peptone .............................................................12.5g

Glucose .............................................................11.0g

Sodium acetate..................................................10.0g

Yeast extract........................................................5.5g

KH2PO4.............................................................0.25g

K2HPO4.............................................................0.25g

MgSO4 ................................................................0.1g

MnSO4·4H2O....................................................0.05g

FeSO4·7H2O......................................................0.05g

pH 6.8 ± 0.2 at 25°C

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Adjust pH to 6.8. Autoclave for 10

min at 15 psi pressure–120°C. Aseptically distribute

into sterile tubes or flasks.

Use: For the cultivation and maintenance of Pedio

coccus acidilactici and Bacillus species.