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Medium N

[所属分类:培养基配方] [发布时间:2021-12-1] [发布人:网站管理员2] [阅读次数:] [返回]

Medium N

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................20.0g

Glucose .............................................................20.0g

Yeast nitrogen base without amino acids............6.7g

Casamino acids, vitamin free..............................2.0g

Isoleucine............................................................0.1g

Valine ..................................................................0.1g

Deoxythymidine-5′-monophosphate

solution ................................................... 10.0mL

Deoxythymidine-5′-Monophosphate Solution:

Composition per 10mL:

Deoxythymidine-5′-monophosphate .............15.0mg

Preparation of Deoxythymidine-5′-Monophos

phate Solution: Add deoxythymidine-5′-monophos

phate to distilled/deionized water and bring volume to

10.0mL. Mix thoroughly. Filter sterilize.

Preparation of Medium: Add components, ex

cept deoxythymidine-5′-monophosphate solution, to

distilled/deionized water and bring volume to

990.0mL. Mix thoroughly. Gently heat and bring to

boiling. Autoclave for 15 min at 15 psi pressure–

121°C. Cool to 60°C. Aseptically add 10.0mL of

sterile deoxythymidine-5′-monophosphate solution.

Mix thoroughly. Pour into sterile Petri dishes or dis

tribute into sterile tubes.

Use: For the cultivation and maintenance of Saccha

romyces cerevisiae.