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Minerals Modified Glutamate Agar

[所属分类:培养基配方] [发布时间:2021-12-2] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Lactose..............................................................10.0g

Sodium glutamate .............................................6.35g

NH4Cl .................................................................2.5g

K2HPO4...............................................................0.9g

Sodium formate ................................................0.25g

MgSO4·7H2O......................................................0.1g

L-Aspartic acid................................................0.024g

L-Arginine.........................................................0.02g

L-Cystine...........................................................0.02g

Bromcresol Purple ............................................0.01g

CaCl2·2H2O ......................................................0.01g

Ferric ammonium citrate...................................0.01g

Nicotinic acid...................................................1.0mg

Pantothenic acid...............................................1.0mg

Thiamine..........................................................1.0mg

pH 6.7 ± 0.2 at 25°c

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat and bring to boiling.

Distribute into tubes or flasks. Autoclave for 10 min

at 11 psi pressure–116°C. Pour into sterile Petri dish

es in 20.0mL volumes.

Use: For the cultivation of coliform bacteria from

foods.