培养基配方

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Minerals Modified Medium

[所属分类:培养基配方] [发布时间:2021-12-2] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Lactose..............................................................20.0g

Sodium glutamate .............................................12.7g

NH4Cl .................................................................5.0g

K2HPO4...............................................................1.8g

Sodium formate ..................................................0.5g

MgSO4·7H2O......................................................0.2g

L-Aspartic acid ................................................0.048g

L-Cystine ...........................................................0.04g

L-Arginine .........................................................0.04g

Ferric ammonium citrate...................................0.02g

CaCl2·2H2O ......................................................0.02g

Bromcresol Purple ............................................0.02g

Thiamine..........................................................2.0mg

Nicotinic acid...................................................2.0mg

Pantothenic acid...............................................2.0mg

pH 6.7 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Preparation of Medium: Add NH4Cl to distilled/

deionized water and bring volume to 800.0mL. Add

remaining components and bring volume to 1.0L.

Mix thoroughly. Adjust pH to 6.7. Distribute into

tubes or flasks. Autoclave for 10 min at 10 psi pres

sure–116°C. Check pH after autoclaving. This medi

um is double strength.

Use: For the enumeration of coliform bacteria.