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Modified Campylobacter Blood-Free Selective Agar Base (Modified Campylobacter Charcoal Differential Agar) (Modified CCDA) (BAM M30a)

[所属分类:培养基配方] [发布时间:2021-12-2] [发布人:网站管理员2] [阅读次数:] [返回]

Modified Campylobacter Blood-Free

Selective Agar Base

(Modified Campylobacter Charcoal

Differential Agar)

(Modified CCDA)

(BAM M30a)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per 1012mL:

Agar ..................................................................12.0g

Beef extract.......................................................10.0g

Peptone..............................................................10.0g

NaCl....................................................................5.0g

Charcoal..............................................................4.0g

Casein hydrolysate..............................................3.0g

Yeast extract........................................................2.0g

Sodium deoxycholate..........................................1.0g

FeSO4 ................................................................0.25g

Sodium pyruvate...............................................0.25g

Cefoperazone solution .................................... 4.0mL

Amphotericin B solution................................. 4.0mL

Rifampicin solution......................................... 4.0mL

pH 7.4 ± 0.2 at 25°C

Cefoperazone Solution:

Composition per 10.0mL:

Cefoperazone ..................................................0.037g

Preparation of Cefoperazone Solution: Add

cefoperazone to distilled/deionized water and bring

volume to 10.0mL. Mix thoroughly. Filter sterilize.

Rifampicin Solution:

Composition per 100.0mL:

Rifampicin ........................................................0.25g

Ethanol, absolute........................................... 50.0mL

Preparation of Rifampicin Solution: Add

rifampicin to 50.0mL of ethanol. Mix thoroughly.

Bring volume to 100.0mL with distilled/deionized

water. Filter sterilize.

Amphotericin B Solution:

Composition per 10.0mL:

Amphotericin B...............................................0.005g

Preparation of Amphotericin B Solution:

Add Amphotericin B to distilled/deionized water and

bring volume to 10.0mL. Mix thoroughly. Filter ster

ilize. Can be stored for 1 year at –20°C.

Preparation of Medium: Add components, ex

cept cefoperazone solution, amphotericin B solution,

and rifampicin solution to distilled/deionized water

and bring volume to 1.0L. Mix thoroughly. Gently

heat and bring to boiling. Autoclave for 15 min at 15

psi pressure–121°C. Cool to 45°–50°C. Aseptically

add 10.0mL of sterile cefoperazone solution, 10.0mL

of sterile amphotericin B solution, and 10.0mL of

sterile rifampicin solution. Mix thoroughly. Pour into

sterile Petri dishes or distribute into sterile tubes.

Use: For the cultivation of Campylobacter species.

For the recovery of injured Campylobacter spp. from

foods.

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Modified Campylobacter Blood-Free Selective Agar Base (Modified Campylobacter Charcoal Differential Agar) (Modified CCDA) (BAM M30a)