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MLCB Agar (Mannitol Lysine Crystal Violet Brilliant Green Agar)

[所属分类:培养基配方] [发布时间:2021-12-2] [发布人:网站管理员2] [阅读次数:] [返回]

MLCB Agar

(Mannitol Lysine Crystal Violet

Brilliant Green Agar)

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Agar ..................................................................15.0g

Peptone .............................................................10.0g

Yeast extract........................................................5.0g

L-Lysine·HCl .......................................................5.0g

NaCl....................................................................4.0g

Na2S2O3 ..............................................................4.0g

Mannitol..............................................................3.0g

Beef extract.........................................................2.0g

Ferric ammonium citrate.....................................1.0g

Crystal Violet....................................................0.01g

Brilliant Green ...............................................12.5mg

pH 6.8 ± 0.1 at 25°C

Source: This medium is available as a premixed

powder from Oxoid.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Mix thoroughly. Gently heat while stirring and bring

to boiling. Do not autoclave. Cool to 50°C. Pour into

sterile Petri dishes in 20.0mL volumes.

Use: For the selective isolation and cultivation of

Salmonella species from foods.