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Møller KCN Broth Base

[所属分类:培养基配方] [发布时间:2021-12-2] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Na2HPO4...........................................................5.64g

NaCl....................................................................5.0g

Pancreatic digest of casein..................................1.5g

Peptic digest of animal tissue .............................1.5g

KH2PO4...........................................................0.225g

KCN solution................................................ 0.15mL

pH 7.6 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

KCN Solution:

Composition per 100.0mL:

KCN....................................................................0.5g

Preparation of KCN Solution: Add KCN to

100.0mL of cold distilled/deionized water. Mix thor

oughly and cap. Do not mouth pipette.

Caution: Cyanide is toxic.

Preparation of Medium: Add components, except

KCN solution, to distilled/deionized water and bring

volume to 1.0L. Mix thoroughly. Autoclave for 15 min

at 15 psi pressure–121°C. Cool to room temperature.

Prior to use, add 0.15mL of KCN solution. Mix thor

oughly. Aseptically distribute into sterile tubes.

Use: For the differentiation of Gram-negative

enteric bacteria on the basis of their ability to grow in

the presence of cyanide.