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Møller Decarboxylase Broth

[所属分类:培养基配方] [发布时间:2021-12-2] [发布人:网站管理员2] [阅读次数:] [返回]

山东拓普生物工程有限公司培养基配方 http://www.topbiol.com

Composition per liter:

Amino acid........................................................10.0g

Peptic digest of animal tissue..............................5.0g

Beef extract.........................................................5.0g

Glucose ...............................................................0.5g

Bromcresol Purple ............................................0.01g

Cresol Red .......................................................5.0mg

Pyridoxal..........................................................5.0mg

pH 6.0 ± 0.2 at 25°C

Source: This medium is available as a premixed

powder from BD Diagnostic Systems.

Preparation of Medium: Add components to

distilled/deionized water and bring volume to 1.0L.

Use L-lysine, L-arginine, or L-ornithine. Mix thor

oughly. Gently heat until dissolved. Distribute into

screw-capped tubes in 5.0mL volumes. Autoclave

for 15 min at 15 psi pressure–121°C. A slight precip

itate may form in the ornithine broth.

Use: For the differentiation of Gram-negative enteric

bacteria based on the production of arginine dihydro

lase, lysine decarboxylase, or ornithine decarboxylase.